Coding

Part:BBa_K5437018

Designed by: Yu-Li He   Group: iGEM24_Formosa   (2024-09-28)


IspH converts HMBPP into DMAPP

IspH is an essential enzyme in the methylerythritol phosphate (MEP) pathway, catalyzing the final step that converts 4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP) into a 5:6 ratio of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). This ratio is later adjusted to 3:7 by the enzyme isopentenyl-diphosphate Δ-isomerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None